| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.130789 |
| Chromosome: | chromosome 12 |
| Location: | 7981771 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552100 | (1 of 15) 3.6.5.5 - Dynamin GTPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCTGCCTCCGCCCCCGGTCGCAGGTGAT |
| Internal bar code: | CGGGCCGCGAAAGAGGTTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 99.706 |
| LEAP-Seq n confirming: | 9495 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTCGTGCCCACACCTAAC |
| Suggested primer 2: | GTGGGACTGCTTATGCCCTA |