| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130793 |
| Chromosome: | chromosome 6 |
| Location: | 1796720 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262400 | (1 of 1) K14676 - lysophospholipid hydrolase (NTE, NRE) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAAGGAGGGCGGGTGTGGTGGTGGTGG |
| Internal bar code: | TAGACCTGACGCTAGGCACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 21.875 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCATGGTTGGTAGCCTT |
| Suggested primer 2: | ATACACGCAAACACGCTCAG |