Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.130822 |
Chromosome: | chromosome_17 |
Location: | 3394054 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g723950 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGTTTCGTCTATCCTTGTCGCGTTTTAAG |
Internal bar code: | AACTACAAGACACGGGATGATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 966 |
LEAP-Seq percent confirming: | 98.6686 |
LEAP-Seq n confirming: | 25272 |
LEAP-Seq n nonconfirming: | 341 |
LEAP-Seq n unique pos: | 151 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACCCCAGAACCCAAGAGTA |
Suggested primer 2: | TACAGCACGAACAGTCGAGG |