| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130827 |
| Chromosome: | chromosome 17 |
| Location: | 3978546 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728750 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACCGTTCGCGCGACATCAATCGATGTA |
| Internal bar code: | GCACCCGGCCCAGGGCACATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 301 |
| LEAP-Seq percent confirming: | 99.7174 |
| LEAP-Seq n confirming: | 3881 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGACATACACGAACAGTG |
| Suggested primer 2: | TTGACAAGAGGTACGAGGGC |