Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.130868 |
Chromosome: | chromosome 10 |
Location: | 1315125 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g427550 | (1 of 3) IPR022751//IPR029044 - Alpha-mannosyltransferase // Nucleotide-diphospho-sugar transferases | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAGCCCGGCTATGTCCAGGAGATACCG |
Internal bar code: | CCTCGCCTGTAGTAAGGGGTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 623 |
LEAP-Seq percent confirming: | 77.439 |
LEAP-Seq n confirming: | 127 |
LEAP-Seq n nonconfirming: | 37 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCCCATTCTTCTTGTTTGT |
Suggested primer 2: | GAGTGGTGTGTGCCATTGTC |