| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130868 |
| Chromosome: | chromosome 12 |
| Location: | 8188013 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550800 | REV1 | (1 of 1) K03515 - DNA repair protein REV1 (REV1); Deoxycytidyl transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTTGCGTCACGGCACCACAGCAGCACAC |
| Internal bar code: | GCGTCGCACGCTTGTATGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 82.1126 |
| LEAP-Seq n confirming: | 4462 |
| LEAP-Seq n nonconfirming: | 972 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAACACCACATCAGGAG |
| Suggested primer 2: | ACTCCATCGATCTGTCCCTG |