Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.130871 |
Chromosome: | chromosome_9 |
Location: | 3256793 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g387912 | FAP139 | Flagellar Associated Protein, serine endopeptidase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCTGCCAGCCTCGCATCACCAAGCCTGATT |
Internal bar code: | GCGATCAAGGACGGGCAGGTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 376 |
LEAP-Seq percent confirming: | 98.9464 |
LEAP-Seq n confirming: | 2254 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGTCCTAGCCAGGTGCAGA |
Suggested primer 2: | CTGTGAGCAGCTCGTTCTTG |