| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.130895 |
| Chromosome: | chromosome 6 |
| Location: | 4380189 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278269 | SCC2 | (1 of 1) K06672 - cohesin loading factor subunit SCC2 (SCC2, NIPBL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGTTTGCTTGTCAGAGAGGCAGGCTGC |
| Internal bar code: | CGTATATCCGTCCGCGAGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 99.3171 |
| LEAP-Seq n confirming: | 4363 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAACTCTTGCGTACGGTG |
| Suggested primer 2: | ACCTTGATGTAGGGTGCCAG |