| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130911 |
| Chromosome: | chromosome 3 |
| Location: | 5726904 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188001 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCTCCGTACGCCTCCCACTAGACAGATC |
| Internal bar code: | TAGGGACCGGTGGCGGAACCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 96.2676 |
| LEAP-Seq n confirming: | 8563 |
| LEAP-Seq n nonconfirming: | 332 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGATCCTACGTGACGCTG |
| Suggested primer 2: | CAACATCGCCTTCTCCTAGC |