| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130923 |
| Chromosome: | chromosome 5 |
| Location: | 3044491 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238687 | PHC35 | Pherophorin-chlamydomonas homolog 35; (1 of 1) IPR011993//IPR024616 - Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) // Pherophorin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGCTCGGCGAGGTCGTACGACCTGGCG |
| Internal bar code: | CCGTGCCGTCGGGACCGTCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 976 |
| LEAP-Seq percent confirming: | 99.54 |
| LEAP-Seq n confirming: | 3679 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGATGCATGATCCCTCTG |
| Suggested primer 2: | TGCAAGAGTGATCAGCAACC |