| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130926 |
| Chromosome: | chromosome 6 |
| Location: | 379346 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g251800 | RFC4 | (1 of 1) PTHR11669//PTHR11669:SF17 - REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT // REPLICATION FACTOR C SUBUNIT 4; DNA replication factor C complex subunit 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACCAGTCTTCTGCTTCGAGGAGTCCCCC |
| Internal bar code: | GAGGGGGGATGAAATGGTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 99.7999 |
| LEAP-Seq n confirming: | 1496 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGCTCTATGTCACGCA |
| Suggested primer 2: | CTGTCGAAAGGCATGTTGAA |