| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.130934 |
| Chromosome: | chromosome 1 |
| Location: | 945619 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005200 | TGL2 | (1 of 3) PTHR21493//PTHR21493:SF153 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // PROTEIN T08B1.4, ISOFORM B-RELATED; Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTGCTCATCGACAACACCTACGGCAAG |
| Internal bar code: | ATCAGACGATCAAACGTCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 633 |
| LEAP-Seq percent confirming: | 24.898 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 184 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCAAGTCTCCTATGCCG |
| Suggested primer 2: | CCATTGTACACATGCGCTTC |