| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130940 |
| Chromosome: | chromosome 9 |
| Location: | 4242725 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394436 | (1 of 1) PF03030 - Inorganic H+ pyrophosphatase (H_PPase) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGTGGCTGCCTGAGGGACGCAGGAAAA |
| Internal bar code: | ACGTAGAGTGCACTTCCCCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 81.6351 |
| LEAP-Seq n confirming: | 1378 |
| LEAP-Seq n nonconfirming: | 310 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTACAAGACCACGCAGCA |
| Suggested primer 2: | TGTTTCTGGAAGGCTTTGCT |