| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.130947 |
| Chromosome: | chromosome_7 |
| Location: | 4063551 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g340400 | RSP17 | Radial spoke protein 17 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCAGGCGTCAGCACGCTTAGCTGCTGCGG |
| Internal bar code: | CCGGTGGGGCAGGTTCCGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 99.5083 |
| LEAP-Seq n confirming: | 4452 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCCAATTACCGACTCAT |
| Suggested primer 2: | CACCATTTGACCACTTGTCG |