| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130951 |
| Chromosome: | chromosome 9 |
| Location: | 7711589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415800 | (1 of 1) K16865 - programmed cell death protein 4 (PDCD4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTAGCGGACCCTGGGTCGTCCGTGCTTA |
| Internal bar code: | AGAATCGGGCGCCGTCGGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.7682 |
| LEAP-Seq n confirming: | 3013 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATTACACCCTGTGTGCG |
| Suggested primer 2: | CCCGACGTTTTGAGTTGTTT |