Insertion junction: LMJ.RY0402.130959_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.130959
Chromosome:chromosome 2
Location:5663099
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g109683 (1 of 3) IPR002912//IPR011598 - ACT domain // Myc-type, basic helix-loop-helix (bHLH) domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATCCCGTGTGCTATGCGCTGATTCGCTA
Internal bar code:GACGAGGCAAATGCGGTGTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:225
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTTCTGGCGGACACTAT
Suggested primer 2:CCATGGGATGGTGTGTATCA