| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130959 |
| Chromosome: | chromosome 10 |
| Location: | 2733243 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438350 | LTH3 | LAT2 homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGCAGAAGCAACAGCACCCCTGGCAGA |
| Internal bar code: | GTCGTCCTTGAAACCATACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 99.8845 |
| LEAP-Seq n confirming: | 1729 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTTGAGGCCTAGCATTC |
| Suggested primer 2: | TGTCGTTGGAGTCAGAGACG |