| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.130959 |
| Chromosome: | chromosome 10 |
| Location: | 2733249 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438350 | LTH3 | LAT2 homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGGGGCCGTTAAGAGCTCAGCGGCCGA |
| Internal bar code: | CAATAGACGTTGAGGATATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.5996 |
| LEAP-Seq n confirming: | 5224 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTTGAGGCCTAGCATTC |
| Suggested primer 2: | TGTCGTTGGAGTCAGAGACG |