Insertion junction: LMJ.RY0402.130963_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.130963
Chromosome:chromosome 1
Location:4522453
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g030800 CSN2 COP9 signalosome subunit; (1 of 1) K12176 - COP9 signalosome complex subunit 2 (COPS2, CSN2, TRIP15) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCGACGCAATTACTCGTGTCAGCACGCT
Internal bar code:TGCGCATGGGGGCTGAGGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1005
LEAP-Seq percent confirming:99.287
LEAP-Seq n confirming:1114
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATACGTGATCCAATGAC
Suggested primer 2:AGTTTTGGAGGTACATGGCG