| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.130983 |
| Chromosome: | chromosome 1 |
| Location: | 1668318 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAATTAGCAAGCCATTTAGTGTAGGAA |
| Internal bar code: | AGATCTTGATTGTGTAGAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 937 |
| LEAP-Seq percent confirming: | 98.4985 |
| LEAP-Seq n confirming: | 3280 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGCGGTGTGTACGGTAT |
| Suggested primer 2: | TTTGCACTTTTGCTTGATGC |