| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130984 |
| Chromosome: | chromosome 2 |
| Location: | 1743928 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCAACAAAGCTTTGCTGGGCGTTAAGG |
| Internal bar code: | CCGTCAAAGCATCTAGCACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 99.072 |
| LEAP-Seq n confirming: | 12597 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCAACAATGGCAACAATA |
| Suggested primer 2: | GGGAGACCGGTACTTGTGAA |