| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.130993 |
| Chromosome: | chromosome 12 |
| Location: | 9653984 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553253 | (1 of 2) PF03121 - Herpesviridae UL52/UL70 DNA primase (Herpes_UL52) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCTACGGCGCCCGACACACCGCGTCAC |
| Internal bar code: | TCCGGGGTTGTATACTTCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 99.7837 |
| LEAP-Seq n confirming: | 3690 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCTCCAACAACACCTG |
| Suggested primer 2: | GGTGACGGTGCATACCTCTT |