| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.130997 |
| Chromosome: | chromosome 12 |
| Location: | 9348031 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCGGCTGCACGCCCGCCAGCTCGGGG |
| Internal bar code: | TACCTTCGACCGCTCGGGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 98.7156 |
| LEAP-Seq n confirming: | 4919 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAAGGGCATGTGTGAGT |
| Suggested primer 2: | TGTGTGTTCCAATCCCAAGA |