| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131001 |
| Chromosome: | chromosome_17 |
| Location: | 3915826 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g728400 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTGGCATAGGCACCGCGCCCATGTGTGC |
| Internal bar code: | CCGGATGCTCCCGCCAGGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 99.7172 |
| LEAP-Seq n confirming: | 1058 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCACTAGACCAACCCGAC |
| Suggested primer 2: | GTGTGTGCGTGAGCAGAAAT |