| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131010 |
| Chromosome: | chromosome 12 |
| Location: | 6351485 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537400 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGTAGGTGGGCGTCGGGCCAGCCCGCG |
| Internal bar code: | GATCACTCTTACCCCTGGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 99.8959 |
| LEAP-Seq n confirming: | 5760 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTAAACCATGCTGATG |
| Suggested primer 2: | TCTGCCTCGAGTTCACCTTT |