Insertion junction: LMJ.RY0402.131013_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.131013
Chromosome:chromosome 17
Location:5747050
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g739150 CTPA5,TSP5 (1 of 8) 3.4.21.102 - C-terminal processing peptidase / Tsp protease; putative C-terminal peptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCCCCCACACTCCCACACTCCCACACC
Internal bar code:CTGAACGGGCGCAGACCAAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:785
LEAP-Seq percent confirming:95.491
LEAP-Seq n confirming:14401
LEAP-Seq n nonconfirming:680
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCGACATTAACTTGGTTT
Suggested primer 2:ACACACCCAGACTGAGACCC