| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131016 |
| Chromosome: | chromosome 6 |
| Location: | 1347353 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258650 | PGM6 | Phosphoglycerate mutase-like protein; (1 of 4) PTHR23029:SF1 - FRUCTOSE-2,6-BISPHOSPHATASE TIGAR | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGGGCAGGGGCCCCTTGAGCGAAGGA |
| Internal bar code: | TGAATCACTTTCGTCGAATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 99.5533 |
| LEAP-Seq n confirming: | 3789 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTCCAAGCTCAAACTGC |
| Suggested primer 2: | CTTCCTGTTACCAGCTTCGC |