| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131017 |
| Chromosome: | chromosome 12 |
| Location: | 4817169 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524550 | (1 of 2) PTHR14614//PTHR14614:SF3 - HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGTGCGCAGGTCAGATCTACCTCCCGC |
| Internal bar code: | GCCCGCGTTTTGATTGTCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 7.72025 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 1016 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGATTTACCTCGGCTGCG |
| Suggested primer 2: | TGGTCGATGTAGCAGCAGTC |