| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131038 |
| Chromosome: | chromosome 4 |
| Location: | 1774977 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212150 | (1 of 1) IPR013815//IPR027417 - ATP-grasp fold, subdomain 1 // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGTAGAAGAAATAGGTTTTTCCAATGC |
| Internal bar code: | TCTACCGTATAGTGGACTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1324 |
| LEAP-Seq percent confirming: | 99.695 |
| LEAP-Seq n confirming: | 8173 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGTCTGTGCCCTTGTCC |
| Suggested primer 2: | GGAGGTTCTGGTGTTGCATT |