| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131083 |
| Chromosome: | chromosome 5 |
| Location: | 1884188 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241629 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAACGGCACACACGCCCTTTCTCCAAG |
| Internal bar code: | GGGTAGGCCAGGCTGTGGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 762 |
| LEAP-Seq percent confirming: | 98.0753 |
| LEAP-Seq n confirming: | 1172 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCAATGGAACCCCTTAG |
| Suggested primer 2: | AATTGTCCATGAAGCAAGGG |