Insertion junction: LMJ.RY0402.131093_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.131093
Chromosome:chromosome 16
Location:1752497
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g654950 SRR3 Scavenger receptor cysteine rich (SRCR) protein; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCTACGTCTCCATCCGTACACAAGAAG
Internal bar code:ATCGTCGAGGTGTGCTGGTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:837
LEAP-Seq percent confirming:99.7338
LEAP-Seq n confirming:2623
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AACAACCCTGTATGCCTTGC
Suggested primer 2:ATTCGTCCAGTACGGCAATC