| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131118 |
| Chromosome: | chromosome 12 |
| Location: | 6977545 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560050 | ERM11,ERM12,RVT1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGAACTAGCGGAGCGCAGGCAGCAGAGG |
| Internal bar code: | ATTCCTGCGTCTCAGACGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 99.9021 |
| LEAP-Seq n confirming: | 7143 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGTGCTTCCTTCTTTTC |
| Suggested primer 2: | ACAGCGTGTGGTGTAATGGA |