Insertion junction: LMJ.RY0402.131120_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.131120
Chromosome:chromosome 1
Location:4656428
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g032050 UAA1 UDP-galactose/glucose transporter; (1 of 1) K15275 - solute carrier family 35 (UDP-galactose transporter), member B1 (SLC35B1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGACTGTAGCACCTCTGGACTGCCGTTGT
Internal bar code:AACGCCACGTCGCTCACAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:746
LEAP-Seq percent confirming:98.4085
LEAP-Seq n confirming:10079
LEAP-Seq n nonconfirming:163
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:ATTACGCCGCCTACATGTTC
Suggested primer 2:GGATTGCTGTGTGGAGCTTT