| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131127 |
| Chromosome: | chromosome 3 |
| Location: | 996560 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148201 | (1 of 10) IPR013781 - Glycoside hydrolase, catalytic domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGCCACCAGGGAAGGAAGGAGCAGAAG |
| Internal bar code: | ACCTGGGGTCGCCCGTGCCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 74.8868 |
| LEAP-Seq n confirming: | 11412 |
| LEAP-Seq n nonconfirming: | 3827 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCTGGCATTCAGATAC |
| Suggested primer 2: | GGAGTAGTTGCTGCTCCTGG |