| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131129 |
| Chromosome: | chromosome 1 |
| Location: | 3179162 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019900 | FAP384 | Flagellar Associated Protein 384 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGGTGTGGGCTAGGCTGTTGCTGGGC |
| Internal bar code: | GACGCTAATGTGAGCACGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 99.8705 |
| LEAP-Seq n confirming: | 6171 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTAAGGCGTGTGGCTAT |
| Suggested primer 2: | CTGCCATCCCTACCACCTTA |