| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131138 |
| Chromosome: | chromosome 12 |
| Location: | 49434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483650 | STL1 | (1 of 3) PTHR11584:SF316 - SERINE/THREONINE-PROTEIN KINASE STN7, CHLOROPLASTIC; Ser-Thr protein kinase, paralog of STT7 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTATGGCCAGCCCACCGCTCGGAATGT |
| Internal bar code: | CCGCCACATGAAAGTCAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 982 |
| LEAP-Seq percent confirming: | 83.2627 |
| LEAP-Seq n confirming: | 3338 |
| LEAP-Seq n nonconfirming: | 671 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTAGATACCAGCCCCAGG |
| Suggested primer 2: | GTCCTCATCTCCCTGCACAT |