| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131152 |
| Chromosome: | chromosome 5 |
| Location: | 3312891 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTGTGGGGTGTGGGGTGAGGGTGAAGG |
| Internal bar code: | ATGCAGGATTAACAGCTCCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 92.5181 |
| LEAP-Seq n confirming: | 22901 |
| LEAP-Seq n nonconfirming: | 1852 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTTGTGGAGTGAGGTGG |
| Suggested primer 2: | TGGCTGACTGAACGACTGAC |