Insertion junction: LMJ.RY0402.131160_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.131160
Chromosome:chromosome 10
Location:3471397
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g444850 CAV7 (1 of 2) PF00520//PF08016//PF16905 - Ion transport protein (Ion_trans) // Polycystin cation channel (PKD_channel) // Voltage-dependent L-type calcium channel, IQ-associated (GPHH); Voltage-gated Ca2+ channel, alpha subunit CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATGGACGTGCCACGAGCAGGCTTATTGC
Internal bar code:TGTCTAAAGGTGCATCTACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:819
LEAP-Seq percent confirming:99.7368
LEAP-Seq n confirming:758
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAAGACCGGCATATCCT
Suggested primer 2:GTAACGCCAGGCTGTGACTT