Insertion junction: LMJ.RY0402.131167_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.131167
Chromosome:chromosome 17
Location:4421058
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g731750 CYP740A1,CYP37 Cytochrome P450, CYP26 superfamily; (1 of 1) 1.14.13.77 - Taxane 13-alpha-hydroxylase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGCAACCCCGCACCCGCACCCCCGACG
Internal bar code:CGGTTGTTACTGGGCGCGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:99.3902
LEAP-Seq n confirming:163
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTGACGGGGTTTGAAGTC
Suggested primer 2:GATCAAGATCAAGGGCGGTA