| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131168 |
| Chromosome: | chromosome 12 |
| Location: | 5205355 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527850 | TRA1 | (1 of 1) PTHR10741//PTHR10741:SF5 - TRANSLIN AND TRANSLIN ASSOCIATED PROTEIN X // TRANSLIN-ASSOCIATED PROTEIN X; Translin-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCACCGATCCGTCGCATGACAGCAATT |
| Internal bar code: | GGGTTGACTGATCTGGACTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.5947 |
| LEAP-Seq n confirming: | 7126 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGGTTGTGCGTCTATG |
| Suggested primer 2: | ACCTCGTTATCACGGTGAGG |