Insertion junction: LMJ.RY0402.131178_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.131178
Chromosome:chromosome 10
Location:4789912
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g453807 CTPB1 (1 of 2) PTHR32060:SF5 - PEPTIDASE S41 FAMILY PROTEIN; C-terminal peptidase B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGTTGCCCGGTAGAGCCATTCCTCGGGT
Internal bar code:TTCTAATTTACTGGAAATCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:96.6567
LEAP-Seq n confirming:10032
LEAP-Seq n nonconfirming:347
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCTTGGTCTTGACACGGT
Suggested primer 2:CTATTGCATTGGGTTCGGTT