Insertion junction: LMJ.RY0402.131179_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.131179
Chromosome:chromosome 17
Location:1516309
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g707150 LIC2 Hypothetical protein; (1 of 45) IPR029052 - Metallo-dependent phosphatase-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTGCCAGACCGCCAAGGGTTGGGATGGT
Internal bar code:CGCGACACGACATGGTCCGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1003
LEAP-Seq percent confirming:99.2233
LEAP-Seq n confirming:13797
LEAP-Seq n nonconfirming:108
LEAP-Seq n unique pos:68

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGATAGCGTAGCAGGCTC
Suggested primer 2:AAGTCAGGATGACCCACCAG