| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131182 |
| Chromosome: | chromosome 12 |
| Location: | 8093468 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551550 | (1 of 1) PF00098//PF02689//PF05970//PF13604 - Zinc knuckle (zf-CCHC) // Helicase (Herpes_Helicase) // PIF1-like helicase (PIF1) // AAA domain (AAA_30) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGGCACCACGGGTGCCCGCACGCAGCC |
| Internal bar code: | GGGTACCTGCCATCGTACGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 99.505 |
| LEAP-Seq n confirming: | 402 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGCATGGGGTAAGAACC |
| Suggested primer 2: | GTATTGCTGGTACGGCGTCT |