| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131222 |
| Chromosome: | chromosome 9 |
| Location: | 1931952 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395100 | MKP4 | (1 of 3) K14165 - atypical dual specificity phosphatase [EC:3.1.3.16 3.1.3.48] (K14165); Dual-specificity protein phosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTACAATACACCCACTCACCCCGCTCAT |
| Internal bar code: | TAGCCAACGTATTCCCTGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 99.6833 |
| LEAP-Seq n confirming: | 9758 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATGGACAACTGGACTGG |
| Suggested primer 2: | AGGTATGCGACCGTATGAGG |