| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.131231 |
| Chromosome: | chromosome 16 |
| Location: | 2052378 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657150 | NAT35 | N-acetyltransferase; (1 of 1) PTHR23091:SF253 - GCN5-RELATED N-ACETYLTRANSFERASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACCCACTCACCATGAGTCCTTGACCAC |
| Internal bar code: | GCCCGTAGAGAACGTGGACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 99.8446 |
| LEAP-Seq n confirming: | 2570 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTACGGCATACATTTCT |
| Suggested primer 2: | AGCGTGACAAGCTTTGGTTT |