| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131241 |
| Chromosome: | chromosome 13 |
| Location: | 2561524 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580900 | (1 of 1) K07178 - RIO kinase 1 (RIOK1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCACGCAGGCCCGCATGGCAATGCCGCA |
| Internal bar code: | GGCAGTCGACTTGTGTAGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 94.9601 |
| LEAP-Seq n confirming: | 1545 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATTTTGACCTCCCAGTA |
| Suggested primer 2: | GCACAGAACCATGCAGAAGA |