| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131261 |
| Chromosome: | chromosome 6 |
| Location: | 7790540 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302426 | 3'UTR | ||
| Cre06.g302450 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGAAAAGGCTCTCGCCCACATCCGCGGC |
| Internal bar code: | GACGGGCGGTCAGTGTTGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.4433 |
| LEAP-Seq n confirming: | 5538 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTCCTGACACGGGCTGT |
| Suggested primer 2: | ACCATCGTTGAAGCTCATCC |