| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131266 |
| Chromosome: | chromosome 5 |
| Location: | 1206016 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245350 | (1 of 1) IPR003582//IPR009011 - ShKT domain // Mannose-6-phosphate receptor binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGGCTGGAGGTGCGTGTGTGTGTATGG |
| Internal bar code: | TAAGAATCACCAGGGCGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 99.8333 |
| LEAP-Seq n confirming: | 5390 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTAAGGACCAGCCCAAAC |
| Suggested primer 2: | AACATGTTTGAGGGTCTGGC |