| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.131272 |
| Chromosome: | chromosome 9 |
| Location: | 2929900 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387950 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGACGCTCCTTTTACCCCGGGTCTAGCTG |
| Internal bar code: | GATGCGGGACCAGCACTTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 98.4424 |
| LEAP-Seq n confirming: | 3792 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCCGAGGAGAACAGTC |
| Suggested primer 2: | AGGGTAGGTTCATGTGACGC |