| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131290 |
| Chromosome: | chromosome 8 |
| Location: | 2315023 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370500 | (1 of 1) IPR013024//IPR017939 - Butirosin biosynthesis, BtrG-like // Gamma-glutamylcyclotransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCCACGACGTCCAGGCCGAGCTCGCA |
| Internal bar code: | GATCACGGGGGAATCGTCAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 71.6346 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCGCTTGATTTATCATTT |
| Suggested primer 2: | CTCTAGAGTGGCGTAACCGC |